Expressing:Article Title: Ecto-5'-nucleotidase/CD73 reduces COX-2 expression in activated macrophages.
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.
Article Title: Ecto-5ʹ-nucleotidase/CD73 reduces COX-2 expression in activated macrophages
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.
Purification:Article Title: Ecto-5'-nucleotidase/CD73 reduces COX-2 expression in activated macrophages.
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.
Article Title: Ecto-5ʹ-nucleotidase/CD73 reduces COX-2 expression in activated macrophages
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.
Transformation Assay:Article Title: Ecto-5'-nucleotidase/CD73 reduces COX-2 expression in activated macrophages.
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.
Article Title: Ecto-5ʹ-nucleotidase/CD73 reduces COX-2 expression in activated macrophages
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.
Plasmid Preparation:Article Title: Ecto-5'-nucleotidase/CD73 reduces COX-2 expression in activated macrophages.
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.
Article Title: Ecto-5ʹ-nucleotidase/CD73 reduces COX-2 expression in activated macrophages
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.
SDS Page:Article Title: Ecto-5'-nucleotidase/CD73 reduces COX-2 expression in activated macrophages.
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.
Article Title: Ecto-5ʹ-nucleotidase/CD73 reduces COX-2 expression in activated macrophages
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.
Cell Culture:Article Title: Ecto-5'-nucleotidase/CD73 reduces COX-2 expression in activated macrophages.
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.
Article Title: Ecto-5ʹ-nucleotidase/CD73 reduces COX-2 expression in activated macrophages
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.
Molecular Weight:Article Title: Ecto-5'-nucleotidase/CD73 reduces COX-2 expression in activated macrophages.
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.
Article Title: Ecto-5ʹ-nucleotidase/CD73 reduces COX-2 expression in activated macrophages
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.
Marker:Article Title: Ecto-5'-nucleotidase/CD73 reduces COX-2 expression in activated macrophages.
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.
Article Title: Ecto-5ʹ-nucleotidase/CD73 reduces COX-2 expression in activated macrophages
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.
Staining:Article Title: Ecto-5'-nucleotidase/CD73 reduces COX-2 expression in activated macrophages.
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.
Article Title: Ecto-5ʹ-nucleotidase/CD73 reduces COX-2 expression in activated macrophages
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.
Western Blot:Article Title: Ecto-5'-nucleotidase/CD73 reduces COX-2 expression in activated macrophages.
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.
Article Title: Ecto-5ʹ-nucleotidase/CD73 reduces COX-2 expression in activated macrophages
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.
Positive Control:Article Title: Ecto-5'-nucleotidase/CD73 reduces COX-2 expression in activated macrophages.
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.
Article Title: Ecto-5ʹ-nucleotidase/CD73 reduces COX-2 expression in activated macrophages
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.
Recombinant:Article Title: Ecto-5'-nucleotidase/CD73 reduces COX-2 expression in activated macrophages.
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.
Article Title: Ecto-5ʹ-nucleotidase/CD73 reduces COX-2 expression in activated macrophages
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.
Comparison:Article Title: Ecto-5'-nucleotidase/CD73 reduces COX-2 expression in activated macrophages.
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.
Article Title: Ecto-5ʹ-nucleotidase/CD73 reduces COX-2 expression in activated macrophages
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.
Activity Assay:Article Title: Ecto-5'-nucleotidase/CD73 reduces COX-2 expression in activated macrophages.
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.
Article Title: Ecto-5ʹ-nucleotidase/CD73 reduces COX-2 expression in activated macrophages
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.
Phosphatase Assay:Article Title: Ecto-5'-nucleotidase/CD73 reduces COX-2 expression in activated macrophages.
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) AR TIC LE IN PR ES S was used at a 1:10,000 dilution.
Article Title: Ecto-5ʹ-nucleotidase/CD73 reduces COX-2 expression in activated macrophages
Article Snippet: Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.Primary antibodies were used at 1:1000 dilution in TBS-T containing 3% BSA and incubated on the membranes overnight at 4 °C.. The primary antibodies used in this study were rabbit anti-COX-2 (Cayman Biochemicals, Ann Arbor, MI), rabbit anti-CD73 (SinoBiologicals), rabbit anti-IκB-α (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-phospho-p42/44 MAPK (detecting endogenous levels of p42/44 only when dually phosphorylated at Thr202 and Tyr204 of Erk1 and Thr185 and Tyr187 of Erk2), and rabbit anti-p42/44 MAPK (both from Cell Signaling Tech, Danvers, USA).. For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.For the normalization of protein loaded, mouse anti-β-actin (Sigma-Aldrich) was used at a 1:10,000 dilution.
|
Sino Biological is a verified supplier 
Sino Biological manufactures this product 
